![]() These premium antibodies are lab-validated using strict testing criteria to ensure superior performance in western blotting detection. PrecisionAb Validated Western Blotting Antibodies.The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce. Loading buffers for protein or nucleic acid samples, detergents, ampholytes, and gel-casting buffers.įrom basic to high-voltage capacity, our power supplies provide simple programming in a compact, stackable case. Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color (blue, orange, green, pink) protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and western blotting. Protein Electrophoresis Buffers and Reagents.Our electrophoresis chambers enable rapid, high-resolution protein separation on precast or handcast gels over a variety of different gel sizes. ![]() Choose SDS-PAGE and native PAGE gels, convert to TGX Precast Gels, or choose specialized gel chemistries. Prestained and unstained molecular weight standards for protein electrophoresis applications including SDS-PAGE, western blotting, 2-D PAGE, and isoelectric focusing (IEF).įind the right Bio-Rad protein gel for your application. Protein Ladders and Standards (Markers).Load the following volumes of the ladder on SDS-polyacrylamide gel: - 5 μl per well for mini-gels, 2.Mix thoroughly to ensure the solution is homogeneous.Thaw the ladder either at room temperature or at 37-40 ☌ for a few minutes to dissolve precipitated solids.The ladder is supplied in gel loading buffer and is ready to use. The BLUEstain TM Protein ladder, is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands ( one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. The BLUEstain TM Protein ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245 kDa. The BLUEstain TM 3 prestained protein ladder is designed for monitoring proteins separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). Shipped in 250 ul vials Protein Ladder 10-180 kDaīLUEstain TM 3 prestained protein ladder is a three-color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa. Sizing of proteins on SDS-polyacrylamide gels and Western blots.Monitoring of protein transfer onto membranes during Western blotting.Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency. The PiNK Plus Prestained Protein Ladder contains 11 prestained proteins that resolve into sharp, tight bands in the range of 10-175 kDa. Protein Ladder 11-175 kDaĮasy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye The BLUEstain TM 2 Protein ladder is a three-color protein standard with 13 prestained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Apply more for thicker (> 1.5 mm) or larger gel.1.5~2.5 μl per well for general Western transferring.3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.Note: Second Quantity should not be changed - please contact us for orders more than 6x500 (3 ml total) Complete - protein marker consists of 11 clearly identifiable bandsĬontents: Approximately 0.1~0.4 mg/ml of each protein in the bufffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).Fast - no need for boiling, dilution or the addition of a reducing agent.This protein marker is prestained and designed for monitoring protein separation during SDS-PAGE verification of western transfer efficiency and approximate sizing of proteins. (Protein Marker Prestained Protein Marker)
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